| 02 May 2000 | 1st Round | Cheshire v Lincolnshire | Boughton Hall Avenue, Chester | Results |
| 02 May 2000 | 1st Round | Cornwall v Norfolk | Boscawen Park, Truro | Results |
| 02 May 2000 | 1st Round | Durham Cricket Board v Leicestershire Cricket Board | Eastwood Gardens, Gateshead | Results |
| 02 May 2000 | 1st Round | Gloucestershire Cricket Board v Nottinghamshire Cricket Board | Victoria Ground, Cheltenham | Results |
| 02 May 2000 | 1st Round | Hampshire Cricket Board v Huntingdonshire | Grasmere Road, Cove | Results |
| 02 May 2000 | 1st Round | Herefordshire v Sussex Cricket Board | Stowe Lane, Colwall | Results |
| 02 May 2000 | 1st Round | Hertfordshire v Cambridgeshire | Digswell Park, Welwyn Garden City | Results |
| 02 May 2000 | 1st Round | Ireland v Shropshire | Castle Avenue, Dublin | Results |
| 02 May 2000 | 1st Round | Northamptonshire Cricket Board v Northumberland | County Ground, Northampton | Results |
| 02 May 2000 | 1st Round | Staffordshire v Somerset Cricket Board | Gorway, Walsall | Results |
| 02 May 2000 | 1st Round | Suffolk v Lancashire Cricket Board | Wamil Way, Mildenhall | Results |
| 02 May 2000 | 1st Round | Wales Minor Counties v Buckinghamshire | Pontarddulais Park, Pontarddulais | Results |
| 02 May 2000 | 1st Round | Wiltshire v Scotland | Bemerton Sports Ground, Salisbury | Results |
| 02 May 2000 | 1st Round | Worcestershire Cricket Board v Kent Cricket Board | Chester Road North, Kidderminster | Results |
| 16 May 2000 | 2nd Round | Cambridgeshire v Cumberland | The Avenue Sports Club Ground, March | Results |
| 16 May 2000 | 2nd Round | Derbyshire Cricket Board v Gloucestershire Cricket Board | Town Ground, Heanor | Results |
| 16 May 2000 | 2nd Round | Devon v Staffordshire | The Recreation Ground, Torquay | Results |
| 16 May 2000 | 2nd Round | Dorset v Norfolk | Dean Park, Bournemouth | Results |
| 16 May 2000 | 2nd Round | Durham Cricket Board v Denmark | Park Drive, Hartlepool | Results |
| 16 May 2000 | 2nd Round | Essex Cricket Board v Lancashire Cricket Board | The Cloud County Ground, Chelmsford | Results |
| 16 May 2000 | 2nd Round | Huntingdonshire v Yorkshire Cricket Board | The Parks, Godmanchester | Results |
| 16 May 2000 | 2nd Round | Lincolnshire v Netherlands | Gorse Lane, Grantham | Results |
| 16 May 2000 | 2nd Round | Middlesex Cricket Board v Wiltshire | John Walker's Ground, Southgate | Results |
| 16 May 2000 | 2nd Round | Northumberland v Bedfordshire | Osborne Avenue, Jesmond | Results |
| 16 May 2000 | 2nd Round | Shropshire v Surrey Cricket Board | Priorslee Road, Shifnal | Results |
| 16 May 2000 | 2nd Round | Sussex Cricket Board v Berkshire | Horntye Park, Hastings | Results |
| 16 May 2000 | 2nd Round | Wales Minor Counties v Oxfordshire | Sophia Gardens, Cardiff | Results |
| 16 May 2000 | 2nd Round | Warwickshire Cricket Board v Kent Cricket Board | Swans Nest Lane, Stratford-upon-Avon | Results |
| 21 Jun 2000 | 3rd Round | Berkshire v Durham | Finchampstead Park, Finchampstead | Results |
| 21 Jun 2000 | 3rd Round | Cumberland v Kent | Edenside, Carlisle | Results |
| 21 Jun 2000 | 3rd Round | Derbyshire Cricket Board v Derbyshire | Racecourse Ground, Derby | Results |
| 21 Jun 2000 | 3rd Round | Devon v Surrey | The Maer Ground, Exmouth | Results |
| 21 Jun 2000 | 3rd Round | Dorset v Glamorgan | Dean Park, Bournemouth | Results |
| 21 Jun 2000 | 3rd Round | Durham Cricket Board v Northamptonshire | Seat Unique Riverside Ground, Chester-le-Street | Results |
| 21 Jun 2000 | 3rd Round | Essex Cricket Board v Warwickshire | The Toby Howe Cricket Ground, Billericay | Results |
| 21 Jun 2000 | 3rd Round | Kent Cricket Board v Hampshire | Spitfire Ground, St Lawrence, Canterbury | Results |
| 21 Jun 2000 | 3rd Round | Lincolnshire v Lancashire | Sports Ground, Cleethorpes | Results |
| 21 Jun 2000 | 3rd Round | Middlesex v Nottinghamshire | Lord's Cricket Ground, St John's Wood | Results |
| 21 Jun 2000 | 3rd Round | Middlesex Cricket Board v Sussex | John Walker's Ground, Southgate | Results |
| 21 Jun 2000 | 3rd Round | Northumberland v Leicestershire | Osborne Avenue, Jesmond | Results |
| 21 Jun 2000 | 3rd Round | Shropshire v Somerset | St Georges Recreation Ground, Telford | Results |
| 21 Jun 2000 | 3rd Round | Wales Minor Counties v Essex | St Helen's, Swansea | Results |
| 21 Jun 2000 | 3rd Round | Worcestershire v Gloucestershire | Visit Worcestershire New Road, Worcester | Results |
| 21 Jun 2000 | 3rd Round | Yorkshire Cricket Board v Yorkshire | St George's Road, Harrogate | Results |
| 04 Jul 2000 | 3rd Round | Worcestershire v Gloucestershire | Visit Worcestershire New Road, Worcester | Results |
| 05 Jul 2000 | 4th Round | Durham v Hampshire | Seat Unique Riverside Ground, Chester-le-Street | Results |
| 05 Jul 2000 | 4th Round | Kent v Glamorgan | Spitfire Ground, St Lawrence, Canterbury | Results |
| 05 Jul 2000 | 4th Round | Lancashire v Essex | Emirates Old Trafford, Manchester | Results |
| 05 Jul 2000 | 4th Round | Leicestershire v Gloucestershire | Uptonsteel County Ground, Leicester | Results |
| 05 Jul 2000 | 4th Round | Middlesex v Somerset | John Walker's Ground, Southgate | Results |
| 05 Jul 2000 | 4th Round | Northamptonshire v Yorkshire | County Ground, Northampton | Results |
| 05 Jul 2000 | 4th Round | Surrey v Sussex | The Kia Oval, Kennington | Results |
| 05 Jul 2000 | 4th Round | Warwickshire v Derbyshire | Edgbaston, Birmingham | Results |
| 25 Jul 2000 | Quarter-Final | Middlesex v Hampshire | Lord's Cricket Ground, St John's Wood | Results |
| 25 Jul 2000 | Quarter-Final | Warwickshire v Glamorgan | Edgbaston, Birmingham | Results |
| 26 Jul 2000 | Quarter-Final | Gloucestershire v Northamptonshire | Seat Unique Stadium, Bristol | Results |
| 26 Jul 2000 | Quarter-Final | Surrey v Lancashire | The Kia Oval, Kennington | Results |
| 12 Aug 2000 | Semi-Final | Warwickshire v Hampshire | Edgbaston, Birmingham | Results |
| 13 Aug 2000 | Semi-Final | Gloucestershire v Lancashire | Seat Unique Stadium, Bristol | Results |
| 26 Aug 2000 | Final | Gloucestershire v Warwickshire | Lord's Cricket Ground, St John's Wood | Results |
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