| 17 May 1965 | | Lancashire Second XI v Northumberland | Emirates Old Trafford, Manchester | Results |
| 24 May 1965 | | Warwickshire Second XI v Durham | Edgbaston Foundation Community Sports Ground, Birmingham | Results |
| 26 May 1965 | | Cambridgeshire v Lincolnshire | The Avenue Sports Club Ground, March | Results |
| 26 May 1965 | | Lancashire Second XI v Cheshire | Emirates Old Trafford, Manchester | Results |
| 26 May 1965 | | Northumberland v Yorkshire Second XI | Osborne Avenue, Jesmond | Results |
| 26 May 1965 | | Staffordshire v Durham | The Sid Jenkins Ground, Little Stoke | Results |
| 27 May 1965 | | Nottinghamshire Second XI v Shropshire | Main Road, Wollaton | Results |
| 31 May 1965 | | Yorkshire Second XI v Lincolnshire | St George's Road, Harrogate | Results |
| 07 Jun 1965 | | Lincolnshire v Bedfordshire | Ross Sports Club Ground, Weelsby Road, Grimsby | Results |
| 07 Jun 1965 | | Northumberland v Durham | Osborne Avenue, Jesmond | Results |
| 07 Jun 1965 | | Yorkshire Second XI v Lancashire Second XI | Queens, Scarborough | Results |
| 09 Jun 1965 | | Cheshire v Staffordshire | Vicarage Road, Northwich | Results |
| 09 Jun 1965 | | Shropshire v Cambridgeshire | High Town, Bridgnorth | Results |
| 16 Jun 1965 | | Cambridgeshire v Bedfordshire | Paradise Sports Ground, Ely | Results |
| 16 Jun 1965 | | Durham v Cumberland | Feethams Cricket Ground, Darlington | Results |
| 16 Jun 1965 | | Shropshire v Lincolnshire | London Road, Shrewsbury | Results |
| 16 Jun 1965 | | Yorkshire Second XI v Northumberland | St George's Road, Harrogate | Results |
| 21 Jun 1965 | | Northumberland v Staffordshire | Osborne Avenue, Jesmond | Results |
| 23 Jun 1965 | | Cumberland v Yorkshire Second XI | Edenside, Carlisle | Results |
| 23 Jun 1965 | | Durham v Staffordshire | Wood Terrace, South Shields | Results |
| 23 Jun 1965 | | Northumberland v Lancashire Second XI | Osborne Avenue, Jesmond | Results |
| 23 Jun 1965 | | Nottinghamshire Second XI v Cambridgeshire | Cricket Field Lane, Retford | Results |
| 23 Jun 1965 | | Shropshire v Warwickshire Second XI | Grove Ground, Market Drayton | Results |
| 28 Jun 1965 | | Yorkshire Second XI v Cumberland | St George's Road, Harrogate | Results |
| 30 Jun 1965 | | Cheshire v Warwickshire Second XI | Vicarage Road, Northwich | Results |
| 30 Jun 1965 | | Hertfordshire v Bedfordshire | Ditchmore Lane, Stevenage | Results |
| 30 Jun 1965 | | Lincolnshire v Shropshire | Abbey Lawn, Bourne | Results |
| 01 Jul 1965 | | Nottinghamshire Second XI v Norfolk | Dale Field, Collingham | Results |
| 05 Jul 1965 | | Shropshire v Somerset Second XI | Reman Services Sports and Social Club Ground, Shewsbury | Results |
| 05 Jul 1965 | | Staffordshire v Norfolk | Boon Hill Road, Bignall End | Results |
| 07 Jul 1965 | | Cheshire v Yorkshire Second XI | Ellerman Line Sports Club, Hoylake | Results |
| 07 Jul 1965 | | Durham v Lancashire Second XI | Blackfyne, Blackhill, Consett | Results |
| 12 Jul 1965 | | Lancashire Second XI v Cumberland | Emirates Old Trafford, Manchester | Results |
| 14 Jul 1965 | | Bedfordshire v Staffordshire | Wardown Park, Luton | Results |
| 14 Jul 1965 | | Cornwall v Somerset Second XI | Trescobeas, Falmouth | Results |
| 14 Jul 1965 | | Hertfordshire v Cambridgeshire | Cricketfield Lane, Bishop's Stortford | Results |
| 14 Jul 1965 | | Lincolnshire v Norfolk | Brumby Hall, Scunthorpe | Results |
| 19 Jul 1965 | | Somerset Second XI v Shropshire | The Cooper Associates County Ground, Taunton | Results |
| 19 Jul 1965 | | Yorkshire Second XI v Durham | Park Avenue Cricket Ground, Bradford | Results |
| 21 Jul 1965 | | Bedfordshire v Cambridgeshire | Wardown Park, Luton | Results |
| 21 Jul 1965 | | Cheshire v Lancashire Second XI | The Kevin McCullagh Oval, Wallasey | Results |
| 21 Jul 1965 | | Nottinghamshire Second XI v Lincolnshire | Elm Avenue, Newark-on-Trent | Results |
| 22 Jul 1965 | | Hertfordshire v Norfolk | Balls Park, Hertford | Results |
| 26 Jul 1965 | | Hertfordshire v Buckinghamshire | Park Avenue, Watford | Results |
| 26 Jul 1965 | | Lancashire Second XI v Durham | Emirates Old Trafford, Manchester | Results |
| 26 Jul 1965 | | Lincolnshire v Cambridgeshire | Lindum Sports Club Ground, Lincoln | Results |
| 26 Jul 1965 | | Norfolk v Staffordshire | County Ground, Lakenham | Results |
| 26 Jul 1965 | | Warwickshire Second XI v Shropshire | Edgbaston, Birmingham | Results |
| 28 Jul 1965 | | Cumberland v Lancashire Second XI | The Playground, Whitehaven | Results |
| 28 Jul 1965 | | Devon v Berkshire | St Thomas Cricket Club Ground, Marsh Barton, Exeter | Results |
| 28 Jul 1965 | | Lincolnshire v Yorkshire Second XI | Lindum Sports Club Ground, Lincoln | Results |
| 28 Jul 1965 | | Norfolk v Buckinghamshire | County Ground, Lakenham | Results |
| 28 Jul 1965 | | Warwickshire Second XI v Cheshire | Edgbaston, Birmingham | Results |
| 30 Jul 1965 | | Cornwall v Devon | Egloshayle Road, Wadebridge | Results |
| 30 Jul 1965 | | Dorset v Berkshire | Sherborne School, Sherborne | Results |
| 30 Jul 1965 | | Suffolk v Buckinghamshire | Ipswich School Ground, Ipswich | Results |
| 02 Aug 1965 | | Bedfordshire v Shropshire | Bedford School Ground, Bedford | Results |
| 02 Aug 1965 | | Berkshire v Buckinghamshire | Church Road, Reading | Results |
| 02 Aug 1965 | | Lancashire Second XI v Yorkshire Second XI | Emirates Old Trafford, Manchester | Results |
| 02 Aug 1965 | | Norfolk v Hertfordshire | County Ground, Lakenham | Results |
| 02 Aug 1965 | | Suffolk v Nottinghamshire Second XI | Town Ground, Felixstowe | Results |
| 02 Aug 1965 | | Wiltshire v Oxfordshire | The County Ground, Swindon | Results |
| 04 Aug 1965 | | Bedfordshire v Lincolnshire | Bedford School Ground, Bedford | Results |
| 04 Aug 1965 | | Berkshire v Dorset | Church Road, Reading | Results |
| 04 Aug 1965 | | Buckinghamshire v Oxfordshire | The Meadow, Chesham | Results |
| 04 Aug 1965 | | Cambridgeshire v Hertfordshire | Spicer's Sports Ground, Sawston | Results |
| 04 Aug 1965 | | Devon v Cornwall | Peverell Park, Plymouth | Results |
| 04 Aug 1965 | | Durham v Northumberland | Eastwood Gardens, Gateshead | Results |
| 04 Aug 1965 | | Norfolk v Nottinghamshire Second XI | County Ground, Lakenham | Results |
| 04 Aug 1965 | | Staffordshire v Cheshire | Trentham Road, Stoke-on-Trent | Results |
| 04 Aug 1965 | | Wiltshire v Somerset Second XI | The County Ground, Swindon | Results |
| 06 Aug 1965 | | Bedfordshire v Hertfordshire | Bedford School Ground, Bedford | Results |
| 06 Aug 1965 | | Buckinghamshire v Berkshire | London Road, High Wycombe | Results |
| 06 Aug 1965 | | Norfolk v Suffolk | County Ground, Lakenham | Results |
| 06 Aug 1965 | | Oxfordshire v Dorset | Smith's Industries Ground, Witney | Results |
| 06 Aug 1965 | | Somerset Second XI v Cornwall | The Cooper Associates County Ground, Taunton | Results |
| 09 Aug 1965 | | Buckinghamshire v Norfolk | Ernest Turner's Sports Ground, High Wycombe | Results |
| 09 Aug 1965 | | Cheshire v Northumberland | Victoria Road, Macclesfield | Results |
| 09 Aug 1965 | | Hertfordshire v Suffolk | Clarence Park, St Albans | Results |
| 09 Aug 1965 | | Oxfordshire v Devon | Roman Way Sports Ground, Oxford | Results |
| 09 Aug 1965 | | Shropshire v Nottinghamshire Second XI | Morda Road, Oswestry | Results |
| 09 Aug 1965 | | Staffordshire v Lancashire Second XI | Oldfields Ground, Uttoxeter | Results |
| 09 Aug 1965 | | Wiltshire v Cornwall | Hardenhuish Park, Chippenham | Results |
| 11 Aug 1965 | | Berkshire v Devon | Home Park, Windsor | Results |
| 11 Aug 1965 | | Buckinghamshire v Suffolk | Ascott Park, Wing | Results |
| 11 Aug 1965 | | Dorset v Cornwall | Redlands Sports Ground, Weymouth | Results |
| 11 Aug 1965 | | Durham v Yorkshire Second XI | Park Drive, Hartlepool | Results |
| 11 Aug 1965 | | Lincolnshire v Nottinghamshire Second XI | Augusta Street, Grimsby | Results |
| 11 Aug 1965 | | Oxfordshire v Wiltshire | Rover Cowley Sports and Social Club Ground, Cowley | Results |
| 11 Aug 1965 | | Staffordshire v Northumberland | Great Chell Cricket Club Ground, Great Chell | Results |
| 13 Aug 1965 | | Berkshire v Wiltshire | St Bartholomew's Grammar School Ground, Newbury | Results |
| 13 Aug 1965 | | Dorset v Devon | Redlands Sports Ground, Weymouth | Results |
| 13 Aug 1965 | | Oxfordshire v Buckinghamshire | The Brakspear Ground, Remenham | Results |
| 16 Aug 1965 | | Buckinghamshire v Hertfordshire | Chalvey Road, Slough | Results |
| 16 Aug 1965 | | Cumberland v Warwickshire Second XI | St George's Road, Millom | Results |
| 16 Aug 1965 | | Norfolk v Lincolnshire | County Ground, Lakenham | Results |
| 16 Aug 1965 | | Oxfordshire v Berkshire | Roman Way Sports Ground, Oxford | Results |
| 16 Aug 1965 | | Shropshire v Bedfordshire | Orleton Park, Wellington | Results |
| 16 Aug 1965 | | Somerset Second XI v Devon | The Cooper Associates County Ground, Taunton | Results |
| 16 Aug 1965 | | Wiltshire v Dorset | Bemerton Sports Ground, Salisbury | Results |
| 16 Aug 1965 | | Yorkshire Second XI v Cheshire | Duke's Park, Bridlington | Results |
| 18 Aug 1965 | | Berkshire v Oxfordshire | Hale's Meadow, Abingdon | Results |
| 18 Aug 1965 | | Cambridgeshire v Nottinghamshire Second XI | Harecroft Road, Wisbech | Results |
| 18 Aug 1965 | | Devon v Dorset | Queens Park, Paignton | Results |
| 18 Aug 1965 | | Durham v Warwickshire Second XI | Carley Hill Ground, Sunderland | Results |
| 18 Aug 1965 | | Northumberland v Cheshire | Osborne Avenue, Jesmond | Results |
| 18 Aug 1965 | | Somerset Second XI v Wiltshire | Agricultural Showgrounds, Frome | Results |
| 18 Aug 1965 | | Staffordshire v Bedfordshire | Danescourt, Wolverhampton | Results |
| 18 Aug 1965 | | Suffolk v Norfolk | Denes Oval, Lowestoft | Results |
| 20 Aug 1965 | | Cornwall v Dorset | Roskear, Camborne | Results |
| 20 Aug 1965 | | Suffolk v Hertfordshire | Town Ground, Felixstowe | Results |
| 20 Aug 1965 | | Wiltshire v Berkshire | London Road, Devizes | Results |
| 23 Aug 1965 | | Devon v Oxfordshire | The County Cricket Ground, Exeter | Results |
| 23 Aug 1965 | | Dorset v Wiltshire | The Recreation Ground, Dorchester | Results |
| 23 Aug 1965 | | Lancashire Second XI v Staffordshire | Emirates Old Trafford, Manchester | Results |
| 23 Aug 1965 | | Nottinghamshire Second XI v Suffolk | Nottinghamshire Sports Ground, Nottingham | Results |
| 25 Aug 1965 | | Cambridgeshire v Shropshire | FP Fenner's Ground, Cambridge | Results |
| 25 Aug 1965 | | Devon v Somerset Second XI | The Recreation Ground, Torquay | Results |
| 25 Aug 1965 | | Dorset v Oxfordshire | The Recreation Ground, Dorchester | Results |
| 25 Aug 1965 | | Cumberland v Durham | Tynefield Park, Penrith | Results |
| 27 Aug 1965 | | Cornwall v Wiltshire | St Clare Ground, Penzance | Results |
| 30 Aug 1965 | | Warwickshire Second XI v Cumberland | Edgbaston, Birmingham | Results |
| 13 Sep 1965 | Challenge Match | Somerset Second XI v Nottinghamshire Second XI | The Cooper Associates County Ground, Taunton | Results |
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