| 05 Apr 2016 | North Division | Durham Second XI v Worcestershire Second XI | Hugill Road, Richmond | Results |
| 28 Apr 2016 | South Division | Middlesex Second XI v Marylebone Cricket Club Young Cricketers | Brunton Memorial Ground, Radlett | Results |
| 03 May 2016 | North Division | Yorkshire Second XI v Unicorns | Shaw Lane, Barnsley | Results |
| 04 May 2016 | North Division | Warwickshire Second XI v Unicorns | Bulls Head Ground, Coventry | Results |
| 05 May 2016 | North Division | Lancashire Second XI v Derbyshire Second XI | Parkgate, Neston | Results |
| 09 May 2016 | North Division | Northamptonshire Second XI v Lancashire Second XI | County Ground, Northampton | Results |
| 11 May 2016 | South Division | Sussex Second XI v Hampshire Second XI | Cricket Field Road, Horsham | Results |
| 19 May 2016 | South Division | Hampshire Second XI v Marylebone Cricket Club Young Cricketers | Utilita Bowl (Nursery), Southampton | Results |
| 23 May 2016 | North Division | Unicorns v Durham Second XI | Burnopfield Cricket Club Ground, Burnopfield | Results |
| 25 May 2016 | North Division | Derbyshire Second XI v Warwickshire Second XI | Repton School Ground, Repton | Results |
| 31 May 2016 | South Division | Somerset Second XI v Glamorgan Second XI | Taunton Vale Sports Club Ground, Taunton | Results |
| 01 Jun 2016 | North Division | Nottinghamshire Second XI v Warwickshire Second XI | Nottinghamshire Sports Ground, Nottingham | Results |
| 02 Jun 2016 | South Division | Middlesex Second XI v Surrey Second XI | Kenton Court Meadow, Sunbury-on-Thames | Results |
| 02 Jun 2016 | South Division | Somerset Second XI v Gloucestershire Second XI | Taunton Vale Sports Club Ground, Taunton | Results |
| 06 Jun 2016 | South Division | Sussex Second XI v Surrey Second XI | Cricket Field Road, Horsham | Results |
| 06 Jun 2016 | North Division | Warwickshire Second XI v Yorkshire Second XI | Station Road, Dorridge | Results |
| 09 Jun 2016 | North Division | Leicestershire Second XI v Nottinghamshire Second XI | Park Road, Loughborough | Results |
| 09 Jun 2016 | North Division | Unicorns v Northamptonshire Second XI | Ledwell Road, Great Tew | Results |
| 10 Jun 2016 | North Division | Leicestershire Second XI v Unicorns | Park Road, Loughborough | Results |
| 15 Jun 2016 | North Division | Unicorns v Worcestershire Second XI | Vicarage Hill, East Challow | Results |
| 16 Jun 2016 | North Division | Yorkshire Second XI v Leicestershire Second XI | Britannia Ground, Pudsey | Results |
| 20 Jun 2016 | North Division | Durham Second XI v Lancashire Second XI | Brandon Lane, Brandon | Results |
| 20 Jun 2016 | South Division | Glamorgan Second XI v Marylebone Cricket Club Young Cricketers | Panteg House, Griffithstown | Results |
| 20 Jun 2016 | South Division | Kent Second XI v Somerset Second XI | Polo Farm Sports Club, Canterbury | Results |
| 20 Jun 2016 | North Division | Northamptonshire Second XI v Derbyshire Second XI | County Ground, Northampton | Results |
| 22 Jun 2016 | South Division | Essex Second XI v Middlesex Second XI | The Toby Howe Cricket Ground, Billericay | Results |
| 27 Jun 2016 | South Division | Sussex Second XI v Kent Second XI | Blackstone Cricket Club Ground, Blackstone | Results |
| 30 Jun 2016 | North Division | Nottinghamshire Second XI v Derbyshire Second XI | Worksop College Ground, Worksop | Results |
| 30 Jun 2016 | South Division | Surrey Second XI v Essex Second XI | London School of Economics Sports Ground, New Malden | Results |
| 04 Jul 2016 | North Division | Derbyshire Second XI v Worcestershire Second XI | Racecourse Ground, Derby | Results |
| 04 Jul 2016 | South Division | Kent Second XI v Glamorgan Second XI | Spitfire Ground, St Lawrence, Canterbury | Results |
| 04 Jul 2016 | North Division | Leicestershire Second XI v Northamptonshire Second XI | Kibworth Cricket Club New Ground, Kibworth | Results |
| 04 Jul 2016 | North Division | Nottinghamshire Second XI v Durham Second XI | Nottinghamshire Sports Ground, Nottingham | Results |
| 07 Jul 2016 | South Division | Hampshire Second XI v Surrey Second XI | Utilita Bowl (Nursery), Southampton | Results |
| 07 Jul 2016 | North Division | Lancashire Second XI v Yorkshire Second XI | The Tyldesleys, Westhoughton | Results |
| 07 Jul 2016 | South Division | Middlesex Second XI v Gloucestershire Second XI | Brunton Memorial Ground, Radlett | Results |
| 11 Aug 2016 | North Division | Lancashire Second XI v Leicestershire Second XI | South Downs Road, Bowdon | Results |
| 15 Jul 2016 | South Division | Sussex Second XI v Middlesex Second XI | Spen Cama Memorial Ground, Fulking | Results |
| 18 Jul 2016 | North Division | Durham Second XI v Northamptonshire Second XI | Seat Unique Riverside Ground, Chester-le-Street | Results |
| 18 Jul 2016 | South Division | Sussex Second XI v Essex Second XI | Blackstone Cricket Club Ground, Blackstone | Results |
| 19 Jul 2016 | South Division | Marylebone Cricket Club Young Cricketers v Somerset Second XI | London Road, High Wycombe | Results |
| 20 Jul 2016 | South Division | Glamorgan Second XI v Hampshire Second XI | Spytty Park, Newport | Results |
| 22 Jul 2016 | South Division | Gloucestershire Second XI v Surrey Second XI | Victoria Ground, Cheltenham | Results |
| 25 Jul 2016 | North Division | Derbyshire Second XI v Yorkshire Second XI | Raynesway, Alvaston | Results |
| 25 Jul 2016 | South Division | Hampshire Second XI v Kent Second XI | Utilita Bowl (Nursery), Southampton | Results |
| 25 Jul 2016 | South Division | Marylebone Cricket Club Young Cricketers v Essex Second XI | London Road, High Wycombe | Results |
| 25 Jul 2016 | South Division | Somerset Second XI v Surrey Second XI | North Parade, Bath | Results |
| 25 Jul 2016 | North Division | Worcestershire Second XI v Lancashire Second XI | Worcester Royal Grammar School Ground, Flagge Meadow, Worcester | Results |
| 26 Jul 2016 | South Division | Glamorgan Second XI v Sussex Second XI | Croft-y-Genau Road, St Fagans | Results |
| 01 Aug 2016 | North Division | Worcestershire Second XI v Nottinghamshire Second XI | Visit Worcestershire New Road, Worcester | Results |
| 05 Aug 2016 | South Division | Essex Second XI v Hampshire Second XI | Hare Field, Coggeshall | Results |
| 04 Aug 2016 | South Division | Marylebone Cricket Club Young Cricketers v Gloucestershire Second XI | Shenley Sports Ground, Shenley | Results |
| 05 Aug 2016 | South Division | Gloucestershire Second XI v Kent Second XI | Victoria Ground, Cheltenham | Results |
| 08 Aug 2016 | South Division | Gloucestershire Second XI v Glamorgan Second XI | North Parade, Bath | Results |
| 08 Aug 2016 | North Division | Warwickshire Second XI v Leicestershire Second XI | Bulls Head Ground, Coventry | Results |
| 08 Aug 2016 | North Division | Yorkshire Second XI v Durham Second XI | Clifton Park, York | Results |
| 10 Aug 2016 | North Division | Worcestershire Second XI v Warwickshire Second XI | Worcester Royal Grammar School Ground, Flagge Meadow, Worcester | Results |
| 11 Aug 2016 | South Division | Essex Second XI v Somerset Second XI | Star Stile, Halstead | Results |
| 12 Aug 2016 | South Division | Kent Second XI v Middlesex Second XI | The Kent County Cricket Ground, Beckenham | Results |
| 12 Aug 2016 | North Division | Northamptonshire Second XI v Nottinghamshire Second XI | Dolben Cricket Ground, Finedon | Results |
| 19 Aug 2016 | Semi-Final | Somerset Second XI v Leicestershire Second XI | The Cooper Associates County Ground, Taunton | Results |
| 19 Aug 2016 | Semi-Final | Kent Second XI v Lancashire Second XI | The Kent County Cricket Ground, Beckenham | Results |
| 25 Aug 2016 | Final | Lancashire Second XI v Somerset Second XI | Emirates Old Trafford, Manchester | Results |
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