| 18 Apr 2016 | South Division | Middlesex Second XI v Essex Second XI | Brunton Memorial Ground, Radlett | Results |
| 18 Apr 2016 | North Division | Northamptonshire Second XI v Leicestershire Second XI | County Ground, Northampton | Results |
| 18 Apr 2016 | North Division | Warwickshire Second XI v Nottinghamshire Second XI | Edgbaston Foundation Community Sports Ground, Birmingham | Results |
| 19 Apr 2016 | South Division | Kent Second XI v Marylebone Cricket Club Young Cricketers | The Kent County Cricket Ground, Beckenham | Results |
| 25 Apr 2016 | North Division | Derbyshire Second XI v Leicestershire Second XI | Barrow Turn, Swarkestone | Results |
| 25 Apr 2016 | South Division | Hampshire Second XI v Somerset Second XI | Utilita Bowl (Nursery), Southampton | Results |
| 25 Apr 2016 | North Division | Lancashire Second XI v Warwickshire Second XI | Emirates Old Trafford, Manchester | Results |
| 25 Apr 2016 | South Division | Middlesex Second XI v Marylebone Cricket Club Young Cricketers | Brunton Memorial Ground, Radlett | Results |
| 02 May 2016 | North Division | Lancashire Second XI v Derbyshire Second XI | Moor Park, Great Crosby | Results |
| 02 May 2016 | South Division | Somerset Second XI v Sussex Second XI | Taunton Vale Sports Club Ground, Taunton | Results |
| 03 May 2016 | South Division | Surrey Second XI v Glamorgan Second XI | Woodbridge Road, Guildford | Results |
| 04 May 2016 | North Division | Leicestershire Second XI v Worcestershire Second XI | Doncaster Close, Oakham School | Results |
| 09 May 2016 | South Division | Gloucestershire Second XI v Essex Second XI | Longwood, Bristol | Results |
| 09 May 2016 | South Division | Marylebone Cricket Club Young Cricketers v Surrey Second XI | London Road, High Wycombe | Results |
| 09 May 2016 | North Division | Nottinghamshire Second XI v Derbyshire Second XI | Nottinghamshire Sports Ground, Nottingham | Results |
| 10 May 2016 | South Division | Glamorgan Second XI v Middlesex Second XI | Marespool, Mumbles | Results |
| 10 May 2016 | North Division | Northamptonshire Second XI v Lancashire Second XI | West Lodge Park, Desborough | Results |
| 16 May 2016 | South Division | Hampshire Second XI v Marylebone Cricket Club Young Cricketers | Utilita Bowl (Nursery), Southampton | Results |
| 16 May 2016 | North Division | Lancashire Second XI v Nottinghamshire Second XI | Aigburth, Liverpool | Results |
| 17 May 2016 | North Division | Warwickshire Second XI v Durham Second XI | Edgbaston Foundation Community Sports Ground, Birmingham | Results |
| 23 May 2016 | South Division | Somerset Second XI v Middlesex Second XI | Taunton Vale Sports Club Ground, Taunton | Results |
| 24 May 2016 | South Division | Essex Second XI v Glamorgan Second XI | Cricketfield Lane, Bishop's Stortford | Results |
| 24 May 2016 | North Division | Worcestershire Second XI v Northamptonshire Second XI | Chester Road North, Kidderminster | Results |
| 25 May 2016 | South Division | Kent Second XI v Gloucestershire Second XI | Polo Farm Sports Club, Canterbury | Results |
| 30 May 2016 | South Division | Middlesex Second XI v Surrey Second XI | Old Deer Park, Richmond | Results |
| 01 Jun 2016 | South Division | Sussex Second XI v Marylebone Cricket Club Young Cricketers | The Saffrons, Eastbourne | Results |
| 06 Jun 2016 | North Division | Leicestershire Second XI v Nottinghamshire Second XI | Leicester Road, Hinckley | Results |
| 07 Jun 2016 | South Division | Essex Second XI v Kent Second XI | The Toby Howe Cricket Ground, Billericay | Results |
| 07 Jun 2016 | North Division | Warwickshire Second XI v Yorkshire Second XI | Edgbaston Foundation Community Sports Ground, Birmingham | Results |
| 07 Jun 2016 | North Division | Worcestershire Second XI v Durham Second XI | War Memorial Ground, Amblecote | Results |
| 13 Jun 2016 | South Division | Middlesex Second XI v Hampshire Second XI | Uxbridge Cricket Club Ground, Uxbridge | Results |
| 13 Jun 2016 | North Division | Yorkshire Second XI v Leicestershire Second XI | Clifton Park, York | Results |
| 14 Jun 2016 | North Division | Durham Second XI v Derbyshire Second XI | Feethams Cricket Ground, Darlington | Results |
| 14 Jun 2016 | South Division | Glamorgan Second XI v Somerset Second XI | Spytty Park, Newport | Results |
| 14 Jun 2016 | South Division | Gloucestershire Second XI v Sussex Second XI | Victoria Ground, Cheltenham | Results |
| 14 Jun 2016 | North Division | Nottinghamshire Second XI v Northamptonshire Second XI | Worksop College Ground, Worksop | Results |
| 20 Jun 2016 | South Division | Hampshire Second XI v Gloucestershire Second XI | May's Bounty, Basingstoke | Results |
| 21 Jun 2016 | North Division | Durham Second XI v Lancashire Second XI | Brandon Lane, Brandon | Results |
| 21 Jun 2016 | South Division | Glamorgan Second XI v Marylebone Cricket Club Young Cricketers | Panteg House, Griffithstown | Results |
| 21 Jun 2016 | South Division | Kent Second XI v Somerset Second XI | Polo Farm Sports Club, Canterbury | Results |
| 21 Jun 2016 | North Division | Northamptonshire Second XI v Derbyshire Second XI | County Ground, Northampton | Results |
| 21 Jun 2016 | North Division | Yorkshire Second XI v Worcestershire Second XI | Queens, Scarborough | Results |
| 27 Jun 2016 | South Division | Glamorgan Second XI v Hampshire Second XI | The Gnoll, Neath | Results |
| 27 Jun 2016 | South Division | Surrey Second XI v Essex Second XI | Peaches Close, Cheam | Results |
| 28 Jun 2016 | North Division | Leicestershire Second XI v Warwickshire Second XI | West Lodge Park, Desborough | Results |
| 28 Jun 2016 | North Division | Marylebone Cricket Club Universities v Lancashire Second XI | Weetwood Playing Fields, Leeds | Results |
| 28 Jun 2016 | South Division | Somerset Second XI v Gloucestershire Second XI | Taunton Vale Sports Club Ground, Taunton | Results |
| 28 Jun 2016 | South Division | Sussex Second XI v Kent Second XI | Blackstone Cricket Club Ground, Blackstone | Results |
| 04 Jul 2016 | North Division | Lancashire Second XI v Yorkshire Second XI | Centre Vale, Todmorden | Results |
| 04 Jul 2016 | South Division | Middlesex Second XI v Gloucestershire Second XI | Brunton Memorial Ground, Radlett | Results |
| 04 Jul 2016 | North Division | Warwickshire Second XI v Marylebone Cricket Club Universities | Edgbaston Foundation Community Sports Ground, Birmingham | Results |
| 05 Jul 2016 | North Division | Derbyshire Second XI v Worcestershire Second XI | Racecourse Ground, Derby | Results |
| 05 Jul 2016 | South Division | Kent Second XI v Glamorgan Second XI | Spitfire Ground, St Lawrence, Canterbury | Results |
| 05 Jul 2016 | North Division | Nottinghamshire Second XI v Durham Second XI | Nottinghamshire Sports Ground, Nottingham | Results |
| 11 Jul 2016 | North Division | Derbyshire Second XI v Warwickshire Second XI | The Copper Yard, Denby Village | Results |
| 11 Jul 2016 | South Division | Hampshire Second XI v Surrey Second XI | Utilita Bowl (Nursery), Southampton | Results |
| 11 Jul 2016 | North Division | Lancashire Second XI v Leicestershire Second XI | Moorside Road, Urmston | Results |
| 11 Jul 2016 | North Division | Marylebone Cricket Club Universities v Worcestershire Second XI | Chester Road North, Kidderminster | Results |
| 11 Jul 2016 | South Division | Sussex Second XI v Middlesex Second XI | Spen Cama Memorial Ground, Fulking | Results |
| 18 Jul 2016 | South Division | Surrey Second XI v Kent Second XI | London School of Economics Sports Ground, New Malden | Results |
| 19 Jul 2016 | North Division | Durham Second XI v Northamptonshire Second XI | Seat Unique Riverside Ground, Chester-le-Street | Results |
| 19 Jul 2016 | North Division | Leicestershire Second XI v Marylebone Cricket Club Universities | West Lodge Park, Desborough | Results |
| 19 Jul 2016 | North Division | Nottinghamshire Second XI v Yorkshire Second XI | Trent College Ground, Long Eaton | Results |
| 19 Jul 2016 | South Division | Sussex Second XI v Essex Second XI | Blackstone Cricket Club Ground, Blackstone | Results |
| 20 Jul 2016 | South Division | Marylebone Cricket Club Young Cricketers v Somerset Second XI | London Road, High Wycombe | Results |
| 25 Jul 2016 | North Division | Leicestershire Second XI v Durham Second XI | West Lodge Park, Desborough | Results |
| 25 Jul 2016 | North Division | Nottinghamshire Second XI v Marylebone Cricket Club Universities | Nottinghamshire Sports Ground, Nottingham | Results |
| 26 Jul 2016 | North Division | Derbyshire Second XI v Yorkshire Second XI | Christchurch Meadows, Belper | Results |
| 26 Jul 2016 | South Division | Hampshire Second XI v Kent Second XI | Newclose County Cricket Ground, Newport | Results |
| 26 Jul 2016 | South Division | Marylebone Cricket Club Young Cricketers v Essex Second XI | London Road, High Wycombe | Results |
| 26 Jul 2016 | North Division | Warwickshire Second XI v Northamptonshire Second XI | Rugby School Ground, Rugby | Results |
| 26 Jul 2016 | South Division | Somerset Second XI v Surrey Second XI | North Parade, Bath | Results |
| 26 Jul 2016 | North Division | Worcestershire Second XI v Lancashire Second XI | Worcester Royal Grammar School Ground, Flagge Meadow, Worcester | Results |
| 27 Jul 2016 | South Division | Glamorgan Second XI v Sussex Second XI | Pen-y-Pound, Abergavenny | Results |
| 02 Aug 2016 | South Division | Essex Second XI v Hampshire Second XI | Hare Field, Coggeshall | Results |
| 01 Aug 2016 | South Division | Marylebone Cricket Club Young Cricketers v Gloucestershire Second XI | Shenley Sports Ground, Shenley | Results |
| 02 Aug 2016 | North Division | Marylebone Cricket Club Universities v Derbyshire Second XI | Weetwood Playing Fields, Leeds | Results |
| 02 Aug 2016 | North Division | Northamptonshire Second XI v Yorkshire Second XI | Fairfield Road, Market Harborough | Results |
| 02 Aug 2016 | North Division | Worcestershire Second XI v Nottinghamshire Second XI | Worcester Royal Grammar School Ground, Flagge Meadow, Worcester | Results |
| 03 Aug 2016 | South Division | Surrey Second XI v Sussex Second XI | London School of Economics Sports Ground, New Malden | Results |
| 08 Aug 2016 | South Division | Essex Second XI v Somerset Second XI | Star Stile, Halstead | Results |
| 08 Aug 2016 | North Division | Marylebone Cricket Club Universities v Northamptonshire Second XI | West Lodge Park, Desborough | Results |
| 09 Aug 2016 | South Division | Gloucestershire Second XI v Glamorgan Second XI | North Parade, Bath | Results |
| 09 Aug 2016 | South Division | Kent Second XI v Middlesex Second XI | The Kent County Cricket Ground, Beckenham | Results |
| 09 Aug 2016 | North Division | Yorkshire Second XI v Durham Second XI | Clifton Park, York | Results |
| 15 Aug 2016 | South Division | Sussex Second XI v Hampshire Second XI | Cricket Field Road, Horsham | Results |
| 22 Aug 2016 | North Division | Warwickshire Second XI v Worcestershire Second XI | Edgbaston Cricket Club Ground, Birmingham | Results |
| 15 Aug 2016 | North Division | Yorkshire Second XI v Marylebone Cricket Club Universities | The Playing Field, Stamford Bridge | Results |
| 16 Aug 2016 | South Division | Gloucestershire Second XI v Surrey Second XI | North Parade, Bath | Results |
| 22 Aug 2016 | North Division | Durham Second XI v Marylebone Cricket Club Universities | Roseworth Terrace, Gosforth | Results |
| 06 Sep 2016 | Final | Durham Second XI v Middlesex Second XI | Seat Unique Riverside Ground, Chester-le-Street | Results |
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