| 09 May 2010 | First Round | South Shore Second XI v Freckleton Second XI | Mad Nook, Blackpool | Results |
| 09 May 2010 | First Round | BAC-EE Preston Second XI v Fulwood and Broughton Second XI | Riverside, Preston | Results |
| 09 May 2010 | First Round | Whittle-le-Woods v New Longton Second XI | Kem Mill Lane, Whittle-le-Woods | Results |
| 09 May 2010 | First Round | Garstang Second XI v Vernon Carus Second XI | Riverside Sports Ground, Garstang | Results |
| 09 May 2010 | First Round | Norcross Second XI v Gregson Lane | HASRA Sports Ground, Thornton Cleveleys | Results |
| 09 May 2010 | First Round | Great Eccleston Second XI v Longridge Second XI | Hall Lane, Great Eccleston | Results |
| 09 May 2010 | First Round | Charnock Richard v Withnell Fold | Mossie Close, Charnock Richard | Results |
| 09 May 2010 | First Round | Wrea Green v Caton | The Green, Wrea Green | Results |
| 09 May 2010 | First Round | Harris Park v Penwortham Second XI | Harrison Park, Wallasey | Results |
| 09 May 2010 | First Round | Whittingham and Goosnargh Second XI v Walton-le-Dale | Guild Park, Whittingham | Results |
| 06 Jun 2010 | Second Round | Whittingham and Goosnargh Second XI v Thornton Cleveleys Second XI | Guild Park, Whittingham | Results |
| 06 Jun 2010 | Second Round | Whittle-le-Woods v Fylde Second XI | Kem Mill Lane, Whittle-le-Woods | Results |
| 06 Jun 2010 | Second Round | Withnell Fold v Fulwood and Broughton Second XI | Withnell Fold, Higher Wheelton | Results |
| 06 Jun 2010 | Second Round | Gregson Lane v Great Eccleston Second XI | Gregson Lane, Hoghton | Results |
| 06 Jun 2010 | Second Round | Kirkham and Wesham v Hoghton Second XI | Woodlands Avenue, Kirkham | Results |
| 06 Jun 2010 | Second Round | Eccleston Second XI v Vernon Carus Second XI | Doctor's Lane, Eccleston | Results |
| 13 Jun 2010 | Second Round | Grimsargh v Wrea Green | The Green, Grimsargh | Results |
| 13 Jun 2010 | Second Round | South Shore Second XI v Penwortham Second XI | Mad Nook, Blackpool | Results |
| 27 Jun 2010 | Quarter-Final | Whittingham and Goosnargh Second XI v Hoghton Second XI | Guild Park, Whittingham | Results |
| 27 Jun 2010 | Quarter-Final | Whittle-le-Woods v Fulwood and Broughton Second XI | Kem Mill Lane, Whittle-le-Woods | Results |
| 27 Jun 2010 | Quarter-Final | Wrea Green v Great Eccleston Second XI | The Green, Wrea Green | Results |
| 27 Jun 2010 | Quarter-Final | Penwortham Second XI v Vernon Carus Second XI | Middleforth Green, Penwortham | Results |
| 25 Jul 2010 | Semi-Final | Vernon Carus Second XI v Whittingham and Goosnargh Second XI | Factory Lane, Penwortham | Results |
| 25 Jul 2010 | Semi-Final | Great Eccleston Second XI v Fulwood and Broughton Second XI | Hall Lane, Great Eccleston | Results |
| 08 Aug 2010 | Final | Fulwood and Broughton Second XI v Vernon Carus Second XI | Highfield, Broughton | Results |
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